A CRISPR RESOURCE FOR INDIVIDUAL, COMBINATORIAL OR MULTIPLEXED GENE KNOCKOUT

CRISPR is a new technology for genetic engineering of human (and many other) cells. This study created a resource for efficiently knocking out 1 or more genes in cell culture so their effects can be studied in a dish.

Abstract

We have combined a machine-learning approach with other strategies to optimize knockout efficiency with the CRISPR/Cas9 system. In addition, we have developed a multiplexed sgRNA expression strategy that promotes the functional ablation of single genes and allows for combinatorial targeting. These strategies have been combined to design and construct a genome-wide, sequence-verified, arrayed CRISPR library. This resource allows single-target or combinatorial genetic screens to be carried out at scale in a multiplexed or arrayed format. By conducting parallel loss-of-function screens, we compare our approach to existing sgRNA design and expression strategies.

28732207*

RELEVANCE TO OC

This is one of the primary methods we use today to study ovarian cancer driver and risk genes, including their associated regulatory elements.

PUBLICATION LINK