A THREE-DIMENSIONAL MICROENVIRONMENT ALTERS PROTEIN EXPRESSION AND CHEMOSENSITIVITY OF EPITHELIAL OVARIAN CANCER CELLS IN-VITRO
Cell culture experiments are typically carried out in a petri-dish, to which the cells adhere and exchange nutrients with the medium. These experiments suffer the drawback that they may not adequately simulate the conditions present in a 3-dimensional tumor space. In this study we show how an emerging technology, 3D cell culture, is applied to 31 patient-derived epithelial ovarian cancer cell lines and characterize the different cell types that and biomarkers associated with 3D vs 2D cultures. We also observed differential sensitivity to therapeutic drugs in the two systems. These findings motivated us to favor these techniques for future studies of ovarian cancer in the laboratory setting.